Synthesis of Headful Packaging Phages Through Yeast Transformation-Associated Recombination
De novo synthesis of phage genomes enables flexible genome modification and simplification.This study explores the synthetic genome assembly cashel tail bag of Pseudomonas phage vB_PaeS_SCUT-S4 (S4), a 42,932 bp headful packaging phage, which encapsidates a terminally redundant, double-stranded DNA genome exceeding unit length.We demonstrate that u